Beilstein J. Org. Chem.2018,14, 1281–1286, doi:10.3762/bjoc.14.109
activity of analogue 22 could be due to its poor internalization or the modification could alter the interaction with tubulin. In order to get an extensive knowledge of the novel analogues, we embarked in docking and modelling studies, herein reported, and internalizationstudies are ongoing in our
Beilstein J. Org. Chem.2012,8, 1788–1797, doi:10.3762/bjoc.8.204
a cell-penetrating peptide.
Keywords: anti-tumor agents; cell-penetrating peptides; drug delivery; internalizationstudies; organometallic complexes; peptides; Introduction
A substantial problem concerning promising drug candidates is often their incapacity to reach their full therapeutic
) were grown in DMEM high glucose (4.5 g/L) containing 1% penicillin/streptomycin and 10% FBS.
Internalizationstudies
For peptide-uptake studies by flow cytometry, cells were seeded in a 48-well plate and grown to 60–70% confluence. After incubation at 37 °C for 1 h with 5(6)-carboxyfluorescein-labeled
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Graphical Abstract
Figure 1:
Flow cytometric uptake studies of carboxyfluorescein-labeled (sC18)2 in HEK-293 (human embryonic ki...